Nikon N-STORM Rendszer
2022. március 20. vasárnap
Címkék:
Mikroszkópok
További részletek az angol nyelvű oldalon olvashatók.
Overview
Component | Type | Description |
---|---|---|
Laser Lines | 405 nm 488 nm 561 nm 642 nm |
50 mW* 200 mW* 300 mW* 330 mW* |
Objectives | 10x 20x 100x Oil |
Plan Fluor NA=0.13 WD=17mm FOV=3215.36um S Plan Fluor NA=0.45 WD=8.2–6.9mm FOV=1290.4um Apo TIRF NA=1.49 WD=0.12mm FOV= ? um |
Microscope Base | Nikon Ti Eclipse | Inverted, fully motorized (stage, filterwheel, turret) |
Stage Adapter | Ti-SH-U | Universal Holder (any microscope slide,35 mm dishes) |
Acquisition Software | NIS Elements AR | Version: 4.51.00 (Build 1143) |
Vibration Isolation Table | Supertech STable | Active pneumatic table |
STORM/TIRF System | ||
TIRF Illuminator | Ti-TIRF-E | variable TIRF angle (1 manuanl/1 motorized direction), 2x/4x focusing lens |
Filter Cubes | HQ Blue** HQ Green** HQ Red** Dual STORM** Far-red STORM** |
EX:370-410nm DM:416nm EM:429-474nm EX:- DM:496nm EM:502-547nm EX:554-568nm DM:575nm EM:557-661nm EX:- DM:500-629/650-757nm EM:500-624/658-755nm EX:- DM:660nm EM:670-760nm |
Camera | Andor iXonEM Ultra 897 | Back illuminated EMCCD 95% Quantum efficiency 512 x 512 pixels (16um pixels) Single photon sensitivity Redout speed:30,10,5 & 1 MHz |
Confocal System | ||
Scanning Head | Nikon C2+ | High precision galvano scanners |
Detector Unit: | C2-DU3 | 3 Standard PMTs |
1st Dichroic Mirror | 405/488/561/640 | Nikon |
Emission Filters | DAPI/CY5(dual) 525/50 nm 594/40 nm |
Semrock EMD/CY5 Custom 239415 Nikon Nikon |
*Nominal output power at laser head
**Custom Filters with Chroma and/or semrock filters
Details
Laser Lines:
The Nikon LU4A laser box contains four lasers as follows:
Wavelength | Type | Manufacturer | Model | Power |
---|---|---|---|---|
408nm* | Solid State | Melles Griot | 56RCS S2780 | 50mW |
488nm* | Solid State | Coherent | Saphire | 200mW |
561nm* | Solid State | Coherent | Saphire | 300mW |
647nm* | Solid State | MPB Communication | VFL-P Series 647nm | 330mW |
*Nominal output power at laser head
Part List:
Component | Type | ID | Description |
---|---|---|---|
Microscope Base | Ti-E Eclipse Ti-PS100W Ti-HUBC/A |
S/N:532257 S/N:2013094 S/N:551490 |
Inverted, fully motorized Controller Base Hub |
xy-Stage | Ti-S-ER Ti-S-CON Ti-S-EJOY |
S/N:661168 S/N:561693 S/N:557697 |
Motorized with encoder Controller Joy-stick |
Stage Adapter | Ti-SH-U | - | Universal Holder (slides,35 mm dishes) |
Piezo Stage | Mad City Labs Nano MCL nano Drive |
S/N:NIK-S1649 S/N:NIK-C1649 |
Piezoelectric stage Controller |
Nosepiece | Ti-ND6-PSF Ti-PFS-CON |
- S/N:594808 |
Motorized nosepiece PSF Controller |
Lasers | |||
Laser Board: | LU4A LU-LR |
S/N:60193 S/N:54488 |
4 laser lines Laser power supply |
408 Laser | Melles Griot 56RCS S2780 | S/N:80466 | 50 mW |
488 Laser | 1. Coherent Saphire 2. Saphire LP USB CDRH |
1. P/N:488-200 CW 1137970 S/N:LDP.1137970.508465 2. P/N:1170412 S/N:LDP1170412.C00464 |
1. 200mW 2. Controller |
561 Laser | 1. Coherent Saphire 2. Saphire LP USB CDRH |
1. P/N:561-300 CW 1255312 S/N:LDP.1255312.175845 2. P/N:1170412 S/N:LDP1170412.C00921 |
1. 300mW 2. Controller |
647 Laser | 1. MPB Communication 2RU-VFL-P-300-647-B1 2. 2RU-VFL-P-300-647-B1 |
1. P/N:MPB-F-04306-113 S/N:1101C037-04 2. S/N:2RU-1123-1725 |
1. 330mW 2. Controller |
Confocal System | |||
Scanning Head | C2+ C2-SH |
S/N:32045 S/N:32045 |
HP galvano scanners Controller |
Detector Unit: | C2-DU3 | S/N:14120 | 3 Standard PMTs |
Widefield System | |||
TIRF Illumination | Ti-N2-SS | S/N:593211 - |
Illumination Unit STORM Lens assembly |
Diascopic Illumination | D-LH/LC Ti-DH Ti-CT-E |
S/N:955374 S/N:602303 - |
100W Halogen Lamp Illumination Pillar Motorized Condensor |
Episcopic Illumination | Intensilight C-HGFIE Controller Ti-FLC-E |
S/N:870068 S/N:620194 - |
1. Enhanced Metal Arc (EmArc) Lamp,Liquid lightguide 2. Fluorescent illumination unit 3. Motorized filter cube turret |
Camera | Andor iXionEM Ultra 897 | P/N:DU-897D-COO-#BV S/N:X-5209 | EMCCD for STORM |