Nikon Fn1 A1R Multiphoton Microscope

Sunday, 20 March, 2022
Tags: Microscopes

The multiphoton confocal microscope enables excitation by simultaneous absorption of two or more near infrared photons by a single fluorescent molecule. Because of the small amount of absorption and scattering by the specimen, the near infrared light used for excitation penetrates deeper into the samples. An additional advantage is that the excited sample volume is diffraction limited in x-y-z, resulting in less photodamage and eliminates the need for a descanned pinhole. The resonant scanner is capable of imaging a wide area at 420 frames per second. The microscope has a large platform stage that can accommodate large samples - cells, tissues or whole organisms.

Overview

Component Type Description
Laser Lines 680 to 1080 Tunable
Ti:Sapphire
Objectives 25x Water Dipping DIC* CFI75 Apo LWD NA=1.1 WD=2mm FOV=?
Microscope Base Nikon FN1 Eclipse Upright
Stage Adapter LN Bridge 500 Luigs&Neumann Slice Mini Bath Chamber
Acquisition Software NIS Elements Version:Version:4.3.00
Vibration Isolation Table Supertech STable Active pneumatic table
Widefield System
Diascopic illumination FN-LH Halogen lamp
Camera

Hamamatsu C7500-51 CCD
Imaging Source DMK NME 41 AU02

NIR Interline CCD
560 × 575 pixels
Multiphoton System
Scanning Head Nikon A1RMP+ High precision galvano and resonant scanners
Detector Unit: A1-GNEF3 2 GaAsP NDD PMTs
2 Standard NDD PMT's
Emission Filters

550/88 nm
641/75 nm
732/68 nm

Nikon 492/SP
Nikon 558/88
Nikon 641/75
Nikon 732/68

Schematic diagram of the lightpath

 

Details

Laser Lines:

Wavelength Type Manufacturer Model Power
680-1080nm
Tunable
Ti:Sapphire Coherent Chameleon Vision II 1.5W at 710nm
3.0W at 800nm
1.35W at 920nm
400mW at 1040nm
180 mW at 1080nm

 

Part List:

Component Type ID Description
Microscope Base FN1 Eclipse
Ti-PS100W/A
Prior NIKRFK
Prior Remote
S/N:842201
S/N:2116345
S/N:R021312273<
S/N:R021312273/td>
Upright Base
Controller
Motorized Z focus
Z focus remote
Nosepiece Mad City Labs Nano-F200-N
Mad City Labs ND1USB161
S/N:MCLS02978
S/N:MCLC02978
1 objective piezo
Piezo Controller
Lasers
Tunable Laser Chameleon Vision II S/N:GDP.1001H.8613 Ti:Sapphire
-
Pumping Laser Coherent Verdi V Series 18V S/N:SDP.1112171.11527 CW, 18W, 532nm
Accessories Coherent T255P 1CR
Coherent MRU X1
P/N:1234080 S/N:A43464
S/N:PPG.1133533.10494
Chiller
Air Compressor Unit
Multiphoton System
Scanning Head A1-SHRM
A1-SHURM
S/N:31308
S/N:31308
Galvano scanners
Controller
Detector Unit A1-GNEF3
A1-NC
S/N:59504
S/N:550034
2 Standard/2 GaAsP NDD PMT-s
S/N:92033
Laser Incident Unit A1-IOU3 S/N:52007 Incident unit
Shutter MP-ES S/N:53036 Shutter
Widefield System
Diascopic Illumination FN-LH
FN1-DP
S/N:857169
-
-
100W Halogen Lamp
Double Port
Episcopic Illumination MP-FN-E
Intensilight C-HGFI
-
S/N:671401
EpiFluor Adapter
Enhanced Metal Arc (EmArc) Lamp
Camera C7500-51
C2741-90
S/N:070085
S/N:540345
Hamamatsu CCD
Hamamatsu Camera Controller
Micromanipulator Stage
Shifting Stage LN V380FM-U S/N:26C100124 Shifting Table (2D)
Stage Adapter LN Bridge 500 P/N:200-100 500 0200 with Slice Mini Bath Chamber
Right Micromanipulator Mini 25 XR
Mini 25 YR
Mini 25 ZR
S/N:14A100640
S/N:14B100588
S/N:14C100633
Luigs&Neumann Junior 4LE-InVivo(3axis Mini 25)
X/Y Controllers LN SM7SN S/N:610-S1407 Luigs&Neumann Controllers
Remote Controllers LN TAS 7 S/N:500-T1413 Luigs&Neumann Remote controllers
Electrophysiological Components
Right Electrodes MD 700B
Axon CV-7B
S/N:836952
S/N:-
Molecular Devices Analog Amplifier
Axon Inst. Headstage Right
Digitizer Digidata 1322A S/N:85559 Axon Instruments
Stimulation Isolator World Precision Instruments P/N:53650/VM2
S/N:135595
-
Peristaltic Pump Watson Marlow 400 S/N:K121980 -
Uninterruptable Power Supply
Central Unit Kstar MP SRTII 3kVA S/N:310016538646305900002 Capacity:3000VA/2400W
Battery Type 9Ah/72Vdc
Battery Units 12
SNMP Card SNMP Card DM801 - Network adapter card
Battery Pack Kstar RTII-BR12009
Kstar RTII-BR12009
S/N:310310777658286300001
S/N:310310777658286300001
Battery Type 9Ah/72Vdc
Battery Units 12
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