Nikon N-STORM Rendszer

2022. március 20. vasárnap

Címkék: Mikroszkópok

További részletek az angol nyelvű oldalon olvashatók.

Overview

Component	Type	Description
Laser Lines	405 nm 488 nm 561 nm 642 nm	50 mW* 200 mW* 300 mW* 330 mW*
Objectives	10x 20x 100x Oil	Plan Fluor NA=0.13 WD=17mm FOV=3215.36um S Plan Fluor NA=0.45 WD=8.2–6.9mm FOV=1290.4um Apo TIRF NA=1.49 WD=0.12mm FOV= ? um
Microscope Base	Nikon Ti Eclipse	Inverted, fully motorized (stage, filterwheel, turret)
Stage Adapter	Ti-SH-U	Universal Holder (any microscope slide,35 mm dishes)
Acquisition Software	NIS Elements AR	Version: 4.51.00 (Build 1143)
Vibration Isolation Table	Supertech STable	Active pneumatic table
STORM/TIRF System
TIRF Illuminator	Ti-TIRF-E	variable TIRF angle (1 manuanl/1 motorized direction), 2x/4x focusing lens
Filter Cubes	HQ Blue HQ Green HQ Red Dual STORM Far-red STORM**	EX:370-410nm DM:416nm EM:429-474nm EX:- DM:496nm EM:502-547nm EX:554-568nm DM:575nm EM:557-661nm EX:- DM:500-629/650-757nm EM:500-624/658-755nm EX:- DM:660nm EM:670-760nm
Camera	Andor iXon^EM Ultra 897	Back illuminated EMCCD 95% Quantum efficiency 512 x 512 pixels (16um pixels) Single photon sensitivity Redout speed:30,10,5 & 1 MHz
Confocal System
Scanning Head	Nikon C2+	High precision galvano scanners
Detector Unit:	C2-DU3	3 Standard PMTs
1st Dichroic Mirror	405/488/561/640	Nikon
Emission Filters	DAPI/CY5(dual) 525/50 nm 594/40 nm	Semrock EMD/CY5 Custom 239415 Nikon Nikon

*Nominal output power at laser head
**Custom Filters with Chroma and/or semrock filters

Details

Laser Lines:

The Nikon LU4A laser box contains four lasers as follows:

Wavelength	Type	Manufacturer	Model	Power
408nm*	Solid State	Melles Griot	56RCS S2780	50mW
488nm*	Solid State	Coherent	Saphire	200mW
561nm*	Solid State	Coherent	Saphire	300mW
647nm*	Solid State	MPB Communication	VFL-P Series 647nm	330mW

*Nominal output power at laser head

Part List:

Component	Type	ID	Description
Microscope Base	Ti-E Eclipse Ti-PS100W Ti-HUBC/A	S/N:532257 S/N:2013094 S/N:551490	Inverted, fully motorized Controller Base Hub
xy-Stage	Ti-S-ER Ti-S-CON Ti-S-EJOY	S/N:661168 S/N:561693 S/N:557697	Motorized with encoder Controller Joy-stick
Stage Adapter	Ti-SH-U	-	Universal Holder (slides,35 mm dishes)
Piezo Stage	Mad City Labs Nano MCL nano Drive	S/N:NIK-S1649 S/N:NIK-C1649	Piezoelectric stage Controller
Nosepiece	Ti-ND6-PSF Ti-PFS-CON	- S/N:594808	Motorized nosepiece PSF Controller
Lasers
Laser Board:	LU4A LU-LR	S/N:60193 S/N:54488	4 laser lines Laser power supply
408 Laser	Melles Griot 56RCS S2780	S/N:80466	50 mW
488 Laser	1. Coherent Saphire 2. Saphire LP USB CDRH	1. P/N:488-200 CW 1137970 S/N:LDP.1137970.508465 2. P/N:1170412 S/N:LDP1170412.C00464	1. 200mW 2. Controller
561 Laser	1. Coherent Saphire 2. Saphire LP USB CDRH	1. P/N:561-300 CW 1255312 S/N:LDP.1255312.175845 2. P/N:1170412 S/N:LDP1170412.C00921	1. 300mW 2. Controller
647 Laser	1. MPB Communication 2RU-VFL-P-300-647-B1 2. 2RU-VFL-P-300-647-B1	1. P/N:MPB-F-04306-113 S/N:1101C037-04 2. S/N:2RU-1123-1725	1. 330mW 2. Controller
Confocal System
Scanning Head	C2+ C2-SH	S/N:32045 S/N:32045	HP galvano scanners Controller
Detector Unit:	C2-DU3	S/N:14120	3 Standard PMTs
Widefield System
TIRF Illumination	Ti-N2-SS	S/N:593211 -	Illumination Unit STORM Lens assembly
Diascopic Illumination	D-LH/LC Ti-DH Ti-CT-E	S/N:955374 S/N:602303 -	100W Halogen Lamp Illumination Pillar Motorized Condensor
Episcopic Illumination	Intensilight C-HGFIE Controller Ti-FLC-E	S/N:870068 S/N:620194 -	1. Enhanced Metal Arc (EmArc) Lamp,Liquid lightguide 2. Fluorescent illumination unit 3. Motorized filter cube turret
Camera	Andor iXion^EM Ultra 897	P/N:DU-897D-COO-#BV S/N:X-5209	EMCCD for STORM