Nikon Ni C2 Konfokális Mikroszkóp

2022. március 20. vasárnap

Címkék: Mikroszkópok

További részletek az angol nyelvű oldalon olvashatók.

Overview

Component	Type	Description
Laser Lines	405 nm 488 nm 561 nm 640 nm	100 mW* 100 mW* 20 mW* 100 mW*
Objectives	4x 10x 20x 60x Oil	Plan Fluor NA=0.13 WD=17.2mm FOV=3215.36um Plan Fluor NA=0.3 WD=16mm FOV=1290.4um Plan Apo VC NA=0.75 WD=1mm FOV=645.12um Plan Apo VC NA=1.45 WD=0.13mm FOV=215.04um
Microscope	Nikon Ni-E Eclipse	Upright, fully motorized (stage, filterwheel, turret)
Stage Adapter	Nikon Ni-SA	Standard microscope slides (25×75 mm)
Acquisition Software	NIS Elements AR	Version: 4.51.01 (Build 1146)
Confocal System
Confocal Scanning Head	C2si	High precision galvano scanners
Confocal Detector Unit:	C2-DU3	3 Standard PMTs
Emission Filters	DAPI/CY5(dual) 525/50 nm 595/40 nm	Semrock EMD/CY5 Custom 239415 Nikon Nikon
Widefield System
Camera	Nikon DS-Fi3	CMOS Sensor: 2880x2048 pixels (6.91 × 4.92 mm, 2.4 um pixel size) ; color/emulated mono; QE=68%; 15 fps (2880 × 2048); 30fps (2x2 average / 1440 × 1024)
Illumination	Nikon Intensilight	Enhanced Metal Vapor Lamp
Filter Cubes	DAPI FITC TRIC CY5	EX: 340-380 nm; DM:400 nm; EM:435-485 nm EX: 465-495 nm; DM: 505 nm; EM: 515-555 nm EX: 527-552 nm; DM: 565 nm; EM: 577-632 nm EX:620/60 nm, DM:660 nm EM:700/71 nm

*Nominal output power at laser head

The Nikon LU4A laser box contains four lasers as follows:

Wavelength	Type	Manufacturer	Model	Power	ID
405 nm	Solid State	Coherent	CUBE	100 mW	P/N, S/N:C18072403
488 nm*	Argon Ion	Melles Griot	IMA101010B0S	17.7 mW	35-IMA-410-019, S/N:M5193
561 nm	Solid State	Coherent	SAPPHIRE 561-20 CW CDHR	20 mW	P/N: 1170380, S/N: LDP.1170380.505233
640 nm	Solid State	Coherent	OBIS 640	100 mW	P/N:, S/N: S200720010

*Argon ion lasers have addition emission lines at 458, 477 and 514 nm

Component	Type	ID	Description
Microscope Base	Ni-E Eclipse	S/N:910033	Inverted, fully motorized (stage, filter wheel, turret)
Microscope Controller	Ni-SA	S/N:5400060	-
xy-Stage	Ni-S-E	S/N:980096	Motorized
Stage Adapter	Ni-SA	-	Standard microscope slides (25×75 mm)
Stage Controller	Ti-S-CON	S/N:562510	-
Laser Board:	LU4A	S/N:60598	4 laser lines
Laser Controllers:	35-INA-840-019 Saphire LP USB CDRH	P/N:300-003 S/N:M6063-2 P/N:1170412 S/N:LDP.1170412.C00431	Melles Griot Argon Ion Controller Coherent Sapphire 561nm
Confocal System
Scanning Head	C2si C2-SHS	S/N:33089 S/N:33089	High precision galvano scanners Controller
Detector Unit:	C2-DU3	S/N:14172	3 Standard PMTs
Widefield System
Camera	DS-5Mc	-	5Mp color camera
Diascopic Illumination	Intensilight C-HGFI	S/N:952280	30W Halogen Lamp
Episcopic Illumination	Intensilight C-HGFI	S/N:672778	Enhanced Metal Arc (EmArc) Lamp Liquid lightguide
Fluorescent Attachment	Ni-FLEI EPI	P/N:MBE94100	Manual ND filters
Filter Cube Turret	Ni-FLt6-E	P/N:MBE94300	Motorized
Condensor	Ni-CUD-E	P/N:MBE99800	Motorized