Measurement of type 2 deiodinase (D2) enzyme activity

Although determining the expression level of D2 is a common way to characterise the intracellular activation of TH, this can often be inaccurate due to the complex posttranslational regulation of the enzyme. Uniquely in Hungary, our laboratory routinely measures D2 activity in animal and human samples using ¹²⁵I isotope-labelled substrate. We are also part of the European Thyroid Association consortium for standardizing the measurement of TH metabolism.