Cytation 5 Cell Imaging Multi-Mode Reader

Friday, 24 March, 2023

Cytation™ 5 is a uniquely integrated, confgurable instrument that combines automated digital widefeld microscopy with conventional multi-mode microplate detection to provide phenotypic cellular information and well-based quantitative data.



Imaging modes: Fluorescence, brightfeld, color brightfeld

Detection mode:

Monochromators: FL, Lum., UV-Vis Abs., Time Resolved Fluorescence (TRF)

Filters: FL, TRF, FP, Lum., Alpha

Read method: End point, kinetic, well mode, time-lapse, montage

Labware type: 6- to 384-well plates, microscope slides, Petri dishes, cell culture flasks (T25); Take3™ Micro-Volume Plates

Temperature control: 4-Zone™ incubation to 65 °C with Condensation Control™; Variation: ±0.2 °C at 37 °C

Shaking: Linear, orbital, double orbital

CO2 and O2 control: Gas Controller

Software: Gen5™ Microplate Reader and Imager Software



Imaging Light source: High power LEDs (365nm, 465nm, 590nm, 523nm)

Camera: 16-bit gray scale, Sony CCD, 1.1 megapixel

Filter cubes: 4 user-replaceable fluorescence cubes plus brightfeld channel

DAPI filter cube with 365nm LED

GFP filter cube with 465nm LED

Texas Red filter cube with 590nm LED

Propidium Iodide Filter Cube with 523nm LED

CFP FRET V2 filter cube with 405nm LED

CFP/YFP FRET V2 filter cube with 405nm LED

Objectives: 6 objectives turret. Available Pan Fluorite objectives:

4x (WD 17 NA 0.13)

10x (10 NA 0.17)

20x (6.7 NA 0.45)

40x (WD 2.7 NA 0.6)

Image collection rate: 96 wells, 1 color (DAPI), 4x, 6 minutes; 96 wells, 3 colors, 4x, 12 minutes

Resolution: 0.3µm/pixel at 20x

Automated functions: Autofocus, autoexposure, auto-LED intensity

Autofocus methods: Laser autofocus; image-based autofocus


Fluorescence Intensity


Monochromators: Top: Fluorescein 2.5 pM (0.25 fmol/well 384-well plate) Bottom: Fluorescein 4 pM (0.4 fmol/well 384-well plate)

Filters/mirrors: Fluorescein 0.25 pM (0.025 fmol/well 384-well plate)

Light source: Xenon flash lamp

Wavelength selection:

Double grating monochromators (top and bottom)

Deep blocking bandpass flters/dichroic mirrors (top)

Wavelength range:

Monochromators: 250 – 700 nm (850 nm option)

Filters: 200 – 700 nm (850 nm option)

Dynamic range: 7 decades

Detection system: Two PMTs: (1) for monochromator system, (1) for flter system




Monochromators: 20 amol ATP (flash);

Filters: 10 amol ATP (flash)

Wavelength range: 300 – 700 nm

Dynamic range: >6 decades


Fluorescence Polarization

Sensitivity: 1.2 mP standard deviation at 1nM fluorescein

Wavelength range: 280 – 700 nm (850 nm option)


Time-Resolved Fluorescence


Europium 40 fM with flters (4 amol/well in 384-well plate)

Europium 1200 fM with monos (120 amol/well in 384-well plate)

Light source: Xenon flash lamp

Wavelength range:

Monos: 250 – 700 nm (850 nm option)

Filters: 200 – 700 nm (850 nm option)



Light source: Xenon flash lamp

Wavelength selection: Monochromator

Wavelength range: 230 – 999 nm, 1 nm increment

Bandpass: 4 nm (230 – 285 nm), 8 nm (>285 nm)

Dynamic range: 0 – 4.0 OD


Alpha Detection

Light source: 680 nm laser, 100 mW +/-10%

Wavelength selection: Filter (top only)

Sensitivity: 100 amol LCK peptide (384-well low volume plate)


Reagent Dispensers

Number: 2 syringe pumps

Dispense volume: 5 – 1,000 µL in 1 µL increment

Dead volume: 1.1 mL, 100 µL with back flush

Plate geometry: 6- to 384-well microplates

Dispense precision: <2% at 50 – 200 μL

Dispense accuracy: ±1 μL or 2%