Abberior Instruments Facility Line STED Microscope

Tuesday, 14 June, 2022

Tags: Microscopes

Overview

Component	Type	Description
Laser Lines	405 nm 485 nm 561 nm 640 nm 775 nm STED	50 mW* 1 mW pulsed - 40 MHz* 200 uW pulsed - 40 MHz* 1 mW pulsed - 40 MHz* 2750 mW pulsed - 40 MHz
Objectives	20x 60x Oil 100x Oil	20x UPlanSApo NA=0.75 WD=0.6 mm FOV=1325 um 60x NA=1.42-oil (UPLXAPO60XO) WD=0.15 mm FOV= 441 um 100x NA 1.4-oil (UPLXAPO100XO) WD=0.2 mm FOV= 0.265 um
Microscope Base	Olympus IX83 Inverted Microscope	Inverted, fully motorized (nosepiece, stage, focus, filterwheel, turret)
Z-drift Compensator	ZDC-830 TrueFocus System	Focus search/Continuous mode - 790 nm/240 uW laser diode
Ultrasonic Stage for IX3	IX3-SSU	range: 76 x 52 mm/resolution: 02 um/max.speed: 30 mm/sec
Acquisition Software	Imspector Data Acqusition and Analysis Environment	System S/N: EX192901 Version: 16.3.14278-w2129-win64 Packages: Dymin, FacilityLine, Hardware, Minfield, Rescue
Vibration Isolation Table	Active self leveling frame	1200mm x 900 mm x 700 mm
Diascopic Illumination
Diascopic Illumination	Transmitted light source	12V 100W halogen bulb
Camera	Eyepiece ONLY
Epifluorescence System
Four color widefield Episcopic Illumination	CoolLED pE-2	QuadBand filtercube DAPI/FITC/Cy3/Cy5
Camera	Eyepiece ONLY
STED System
Scanning Head	AI Facility Line/Cambridge Technology	-
	561 nm/640 nm depleted with 775 nm STED laser
Detectors	APDs/Matrix	1st,2nd,4th APDs 3th Matrix detector
Spectral Filters	variable 400 nm - 800 nm range	-
Confocal System
Scanning Head	QUADScan Beam Scanner	ScanLens-free design / 4 galvo scanners / Image rotation / Image tilting in 3D / 80 um x 80 um / 16000 x 16000 pix (256 Mpixels)
Pinholes	Pinhole wheel with fix-sized pinholes	Pinhole sizes:
Spectral Filters	variable 400 nm - 800 nm range	-
Detectors	APDs/Matrix	1st,2nd,4th APDs 3th Matrix array detector
FLIM module
-	SPC-150N, SPC-150NX, SPC-150NXX TCSPC/FLIM Modules	Minimum time channel width: 203 fs / Time resolution: 1.1 ps / 10 Mhz Saturated Count Rate /

Details:

Laser lines:

Wavelength	Type	Manufacturer	S/N	Power
405nm	Solid State	Laser Module 405	Rev. 1.1, S/N:LM192503	50 mW continuous
485nm	Solid State	PDL-T Laser Head 488	Rev. 4.4, S/N:PDL-T 185004	1 mW pulsed - 40 MHz
561nm	Solid State	PDL-T Laser Head 561	Rev. 4.4, S/N:PDL-T 184703	120 uW pulsed - 40 MHz - 120 ps
640nm	Solid State	PDL-T Laser Head 640	Rev. 4.4, S/N:PDL-T 192101	1 mW pulsed - 40 MHz
775nm STED	Solid State			2750 mW pulsed - 25-40 MHz

Components	Type	ID	Description
Microscope Base	IX83 Inverted Microscope - Two-Deck System/IX83P2ZF Olypus I3-TPC Control Pad	S/N: OH85107 S/N: OH85107	Inverted, fully motorized Touchscreen
xy-Stage/Focusing	Olympus IX3-SSU Olympus U-MCZ	S/N: OK46064 S/N: OE42826	Controller/XY/Focus
Continuous Focus	IX3-ZDC2-830 TrueFocus	S/N: 0G4984
STED System Confocal System	4C Facility Line STED Microscope	S/N: FC215001
Epifluorescence System	CoolLED pE-2 light source Collimating Adaptor	S/N: AF1655 S/N: AA101596