Nikon STORM2 Microscope

Sunday, 20 March, 2022

Tags: Microscopes

Starting the STORM2 system

Overview

Component	Type	Description
Laser Lines	405 nm 488 nm 561 nm 647 nm	67 mW* 120 mW* 120 mW* 400 mW* Currently unavailable!
Objectives	10x 20x 40x 100x Oil 100x Sil	Plan Fluor NA=0.3 WD=16.00mm FOV=3215.36um CFI Plan Apo VC NA=0.75 WD=1.00mm FOV=1290.4um CFI Apochromat Lambda S 40x/1.25 WI NA=1.25 WD=0.18mm FOV(full)= 840um CFI SR HP Apochromat TIRF 100XAC Oil NA=1.49 WD=0.12mm FOV(full)= 310um CFI SR HP Plan Apochromat Lambda S 100XC Sil NA=1.35 WD=0.3mm FOV= ? um
Microscope Base	Nikon Eclipse Ti2	Inverted, fully motorized (stage, filterwheel, turret)
Stage Adapter	Ti-SH-U	Universal Holder (any microscope slide,35 mm dishes)
Acquisition Software	NIS Elements AR	Version: 5.21.00
Vibration Isolation Table	Supertech STable	Active pneumatic table
DIC
Diascopic Illumination	Objective /DIC-slider	20x CFI Plan Apo VC NA=0.75 / 20x 100x CFI SR HP Apochromat TIRF AC Oil / 100xI-R
Camera	HAMAMATSU C13440-20CU ORCA-Flash4.0 V3	sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm)
Epifluorescence System
Episcopic Illumination	CoolLED pE-4000	Excitation Lines Recommended wavelengths/filter cubes and Possible wavelength combinations
Camera	HAMAMATSU C13440-20CU ORCA-Flash4.0 V3	sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm)
STORM/TIRF System
STORM/TIRF Illuminator	TI2-LA-NS2	variable TIRF angle (2 motorized directions), 2x/4x/8x focusing lens
Filter Cubes	DIC-analizator Laser-FITC STORM Cont. STORM CoolLED DAPI/FITC/TxRed CoolLED CFP/YFP/mCherry**	EX:387-417/483-494/557-570/636-661 DM:420-481/497-553/575-628/667-792 EM:422-478/502-549/581-625/674-786 EX:378/57_474/30_575/31 DM:414-450/499-546/604-850 EM:432/40_523/50_702/196 EX:475/27_543/27_702/201 DM:350-450/498-520/567-589 EM:438/29_509/26_578/25
Camera	HAMAMATSU C13440-20CU ORCA-Flash4.0 V3	sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm)
Confocal System
Scanning Head	Nikon C2+	High precision galvano scanners
Detector Unit:	C2-DU4 C2-DUT	3 Standard PMTs 1 Diascopic PMT detector
1st Dichroic Mirror	405/488/561/640	Nikon
Emission Filters	DAPI/CY5(dual) 427-463 nm/662LP 525/50 nm 594/40 nm	Semrock D/CY5 Custom 239415 Nikon Nikon

*Nominal output power at laser head
**Custom Filters with Chroma and/or semrock filters

Details

Laser Lines:

The Nikon LU4A laser box contains four lasers as follows:

Wavelength	Type	Manufacturer	Model	Power
405nm*	Solid State	Spectra-Physics	N1246-70021-CCI	67mW
488nm*	Solid State	Coherent	Sapphire 488-120 FP	120mW
561nm*	Solid State	Coherent	Sapphire 561-120 FP	120mW
647nm*	Solid State	MPB Communications Inc.	VFL-P-400-647-OEM2-B1	400mW

*Nominal output power at laser head

*Laser power of STORM2 vs Ni-E C2

Part List:

Component	Type	ID	Description
Microscope Base	Nikon Eclipse Ti2-E Ti2-CTRE Ti2-LA-CTR	S/N:541848 S/N:451269 S/N:558145	Inverted, fully motorized Controller
xy-Stage/Focusing	Ti2-S-JS	S/N:128473	Controller/Joystick/Focus
Stage Adapter	Ti-SH-U	-	Universal Holder (slides,35 mm dishes)
Piezo Stage	Mad City Labs Nano MCL nano Drive	S/N:NIK-S1649 S/N:NIK-C1649	Piezoelectric Stage Controller
Nosepiece	- -	- -	Motorized nosepiece PFS Controller
Lasers
Laser Board Laser Controller	LU-NV-0 LASER UNIT LU-C	S/N:60379 S/N:465253	4 laser lines Laser power supply
405 Laser	Spectra-Physics N1246-70021-CCI
488 Laser	Coherent Sapphire 488-120 FP
561 Laser	Coherent Sapphire 561-120 FP
647 Laser	MPB Communications Inc. VFL-P-400-647-OEM2-B1
Confocal System
NATIONAL INSTRUMENTS PXI Chassis	PXI-1033	S/N:3151D37	*
Scanning Head	C2+ C2-SH	S/N: S/N:432006	HP galvano scanners Controller
Detector Unit:	C2-DU3	S/N:14656	3 Standard PMTs 1 Diascopic PMT detector
Widefield System
STORM/TIRF Illumination	TI2-LA-NS2	S/N:592561	Illumination Unit STORM Lens assembly
Diascopic Illumination	D-LH/LC Ti-DH Ti-CT-E	S/N:955374 S/N:602303 -	100W Halogen Lamp Illumination Pillar Manual/Intelligent Condensor
Episcopic Illumination	Intensilight C-HGFIE Controller -	S/N:870068 S/N:620194 -	1. Enhanced Metal Arc (EmArc) Lamp,Liquid lightguide 2. Fluorescent illumination unit 3. Motorized filter cube turret
Episcopic Illumination	CoolLED pE-4000	S/N:CP0395	Liquid lightguide
Camera	HAMAMATSU C13440-20CU ORCA-Flash4.0 V3	S/N:301746	sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm)
Cage Incubator/Temperature Unit/Touch screen interface	OKOLAB H201 T AIR HEATER/OKO-TOUCH	?/S/N:032-1787/S/N:748-3282	Gas type: 5%CO2 Temp. accuracy: ±0.3℃ at each heater surface Forced humidification by heating Bath Unit
Stage Top Incubator	TOKAI HIT/Model: STXF-TIZWX-SET	S/N:180470
Peristaltic Pump	WATSON MARLOW ALITEA Auto cased pump 50rpm	S/N:692607