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Molecular cloning and protein work
Sunday, 20 March, 2022
Our key research focus centers on the function of distinct molecular players of the endocannabinoid system and other lipid signaling pathways in the nervous system.
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Methods
Field / type of information: Cikk
Research group / Central unit: Molecular Neurobiology Research Group
Immunohistochemistry
Sunday, 20 March, 2022
To uncover the precise regional, cellular and subcellular localization of distinct proteins, we use three complementary methods of immunohistochemistry.
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Methods
Field / type of information: Cikk
Research group / Central unit: Molecular Neurobiology Research Group
Immunocytochemistry and live-cell imaging
Sunday, 20 March, 2022
We use several cell culture preparations and express various constructs in these cells to study the operation and function of endocannabinoid-mediated or endo-cannabinoid-related signaling pathways.
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Methods
Field / type of information: Cikk
Research group / Central unit: Molecular Neurobiology Research Group
In utero electroporation
Sunday, 20 March, 2022
To label specific neuronal populations or to regulate gene expression levels in given cell types (either by overexpression or by siRNA silencing), in utero electroporation is an emerging approach.
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Methods
Field / type of information: Cikk
Research group / Central unit: Molecular Neurobiology Research Group
Patch-clamp electrophysiology
Sunday, 20 March, 2022
To investigate the functional significance of the signaling pathways, which we have uncovered in our molecular biology and anatomy experiments, we perform whole-cell patch-clamp recordings in acute brain slice preparations.
Tags:
Methods
Field / type of information: Cikk
Research group / Central unit: Molecular Neurobiology Research Group