In situ hybridization with radioisotopic and digoxigenin-labeled cRNA probes

Human brain tissues collected shortly post mortem are compatible with in situ hybridization experiments. Figures illustrate autoradiographic in situ hybridization signal to RF-amide related peptide (RFRP) mRNA in the hypothalamic periventricular nucleus (left) and non-isotopic in situ hybridization signal to GnRH mRNA in the human putamen (right).

Skrapits et al. Elife 2021 doi: 10.7554/eLife.67714