In situ hybridization with radioisotopic and digoxigenin-labeled cRNA probes

Sunday, 20 March, 2022

In situ hybridization detection of neurons expressing RF-amide related peptide and GnRH mRNAs in the human hypothalamus


Human brain tissues collected shortly post mortem are compatible with in situ hybridization experiments. Figures illustrate autoradiographic in situ hybridization signal to RF-amide related peptide (RFRP) mRNA in the hypothalamic periventricular nucleus (left) and non-isotopic in situ hybridization signal to GnRH mRNA in the human putamen (right).


Skrapits et al. Elife 2021 doi: 10.7554/eLife.67714