LCM-Seq protocol developed for whole-transcriptome profiling of spatially resolved cholinergic neuron populations
Detection of estrogen receptor-β in the cell nucleus of human GnRH neurons
Postmenopausal hypertrophy of human kisspeptin neurons
Detection of neuropeptide colocalization in human hypothalamic neurons
Ultrastructural detection of synapses between kisspeptin neurons of the human hypothalamus
In situ hybridization detection of neurons expressing RF-amide related peptide and GnRH mRNAs in the human hypothalamus
Analysis of kisspeptin neuron morphology in the post mortem hypothalamus
Studies of glutamatergic markers (VGLUT1 and VGLUT2) in samples of the human mediobasal hypothalamus
Use of whole-cell patch-clamp electrophysiology to study the differential responsiveness of cholinergic neuron populations