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Spatial transcriptomics with LCM-Seq to study fluorescently tagged neurons from transgenic mice

LCM-Seq protocol developed for whole-transcriptome profiling of spatially resolved cholinergic neuron populations


Detection of estrogen receptor-β in the cell nucleus of human GnRH neurons

Morphometry, image analysis

Postmenopausal hypertrophy of human kisspeptin neurons


Immunofluorescent multiple-labeling, confocal microscopy

Detection of neuropeptide colocalization in human hypothalamic neurons


Electron microscopy

Ultrastructural detection of synapses between kisspeptin neurons of the human hypothalamus

In situ hybridization with radioisotopic and digoxigenin-labeled cRNA probes

In situ hybridization detection of neurons expressing RF-amide related peptide and GnRH mRNAs in the human hypothalamus


Random DiI-labeling and 3D-reconstruction of immunolabeled neurons in post mortem brains

Analysis of kisspeptin neuron morphology in the post mortem hypothalamus


RT-qPCR studies of microdissected hypothalamic nuclei

Studies of glutamatergic markers (VGLUT1 and VGLUT2) in samples of the human mediobasal hypothalamus

Whole-cell patch-clamp electrophysiology

Use of whole-cell patch-clamp electrophysiology to study the differential responsiveness of cholinergic neuron populations