Light and electron microscopic immunohistochemistry

 Diaminobenzidine (DAB) and Ni-DAB chromogens are used for single-and double-labeling immunohistochemistry. Immunofluorescence combined with confocal microscopy is used for co-localization studies and for simultaneous localization of more than two peptides/proteins. 

For ultrastructural localization of peptides/proteins, we combine electron-microscopy with single- or double-labeling preembedding immunocytochemistry using DAB, Ni-DAB chromogens and gold-colloids with or without silver intensification.

When necessary, tyramide signal amplification system is used to detect weak signals.