Methods

We generate antibodies against difficult antigens or against antigens that we plan to very frequently detect in our laboratory. 

We use immunocytochemistry to determine neuronal or neuro-glial interactions and the localization of peptides/proteins in neuronal tissues.

To localize and / or quantitate certain type of mRNA in tissue sections, we use in situ hybridization. 

Anterograde- and retrograde tract tracing molecules, like phaseolus vulgaris leucoagglutinin, cholera-toxin β subunit or biotinylated dextrans are used to determine projection fields of neuronal groups and sources of neuronal inputs. 

Together with the Molecular Cell Metabolism Laboratory, we have developed a transgenic mouse model, the Thyroid Hormone Action Indicator (THAI) mouse. 

To determine how the gene expression of specific neuronal populations or peripheral tissues is influenced by physiological or pathological conditions, genetic manipulations or pharmacological treatments, we use TaqMan quantitative PCR. 

To study gene expression of multiple genes in small brain regions or in a certain cell type, we perform laser capture microdissection (LCM). 

To understand the regulation of energy homeostasis, we perform metabolic phenotyping using Phenomaster Metabolic Unit (TSE). 

Electrophysiological properties are key features of neurons.