Applied Methods

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Antibody generation

We generate antibodies against difficult antigens or against antigens that we plan to very frequently detect in our laboratory. 

Light and electron microscopic immunohistochemistry

We use immunocytochemistry to determine neuronal or neuro-glial interactions and the localization of peptides/proteins in neuronal tissues.

Isotopic and fluorescent in situ hybridization

To localize and / or quantitate certain type of mRNA in tissue sections, we use in situ hybridization. 

Neuronal tract-tracing methods

Anterograde- and retrograde tract tracing molecules, like phaseolus vulgaris leucoagglutinin, cholera-toxin β subunit or biotinylated dextrans are used to determine projection fields of neuronal groups and sources of neuronal inputs. 

Examination of tissue specific thyroid hormone action using THAI mouse model

Together with the Molecular Cell Metabolism Laboratory, we have developed a transgenic mouse model, the Thyroid Hormone Action Indicator (THAI) mouse. 

Gene expression analysis using TaqMan quantitative PCR

To determine how the gene expression of specific neuronal populations or peripheral tissues is influenced by physiological or pathological conditions, genetic manipulations or pharmacological treatments, we use TaqMan quantitative PCR. 


Laser capture microdissection

To study gene expression of multiple genes in small brain regions or in a certain cell type, we perform laser capture microdissection (LCM). 

Metabolic Phenotyping and body composition analysis

To understand the regulation of energy homeostasis, we perform metabolic phenotyping using Phenomaster Metabolic Unit (TSE). 

Patch clamp electrophysiology

Electrophysiological properties are key features of neurons.