Antibody generation

Friday, 19 May, 2023

We generate antibodies against difficult antigens or against antigens that we plan to very frequently detect in our laboratory.

We use thyroglobulin-conjugated peptides or recombinant proteins to generate antisera in rats, mice, rabbits or sheep. If necessary, affinity purification is used to increase the specificity of the antisera.

Raised antibodies:

- Affinity purified sheep antibody against histamine (Brain Res. 2003; 969(1-2):70-7. doi: 10.1016/s0006-8993(03)02279-0)

- Affinity purified sheep antibody against TRH (J Comp Neurol. 2009;517(6):825-40. doi: 10.1002/cne.22180)

- Affinity purified mouse antibody against TRH (Brain Res. 2012;1488:72-80. doi: 10.1016/j.brainres.2012.10.010)

- Rat antiserum against mouse proTRH 178-200 (Brain Struct Funct. 2018;223(3):1329-1341. doi: 10.1007/s00429-017-1553-5)

- Sheep antiserum against POMC (Brain Struct Funct. 2018;223(3):1329-1341. doi: 10.1007/s00429-017-1553-5)

- Sheep antiserum against red fluorescent protein (recognizing RFP, mCherry, tdTomato) (Thyroid. 2019;29(12):1858-1868. doi: 10.1089/thy.2019.0357)

- Sheep antiserum against green fluorescent proteins (recognizing GFP, YFP, YPET)