New preprint on bioRxiv: Innovative IHC/LCM-Seq method for transcriptomic profiling of human brain neurons
Our latest study has been published on bioRxiv, presenting and validating a novel IHC/LCM-Seq technique.
This pioneering method enables transcriptome-wide profiling of neuronal populations that can be identified by immunohistochemistry in formaldehyde-fixed human brain tissue sections.
Using IHC/LCM-Seq, we obtained unprecedented insights into the gene expression patterns of key neuronal populations within the hypothalamic arcuate/infundibular nucleus. Specifically, we characterized agouti-related peptide (AgRP) neurons, which stimulate food intake and energy storage, proopiomelanocortin (POMC) neurons, which suppress feeding and promote energy expenditure, as well as kisspeptin (KP) neurons, central regulators of fertility.
Our results demonstrate that IHC/LCM-Seq is a reliable and versatile tool for transcriptomic analyses of spatially defined cell types in the human brain. We believe that this approach will open new avenues for uncovering the molecular foundations of fundamental neurobiological processes and their impact on human health.
